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-Microscopie gebaseerde testen voor high-throughput screening van Host factoren die betrokken zijn in<em> Brucella</em> Infectie van Hela cellen
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-Microscopie gebaseerde testen voor high-throughput screening van Host factoren die betrokken zijn in<em> Brucella</em> Infectie van Hela cellen
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August 05, 2016
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Two assays for microscopy-based high-throughput screening of host factors involved in Brucella infection are described. The entry assay detects host factors required for Brucella entry and the endpoint assay those required for intracellular replication. While applicable for alternative approaches, siRNA screening in HeLa cells is used to illustrate the protocols.
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Casanova, A., Low, S. H., Emmenlauer, M., Conde-Alvarez, R., Salcedo, S. P., Gorvel, J., Dehio, C. Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells. J. Vis. Exp. (114), e54263, doi:10.3791/54263 (2016).
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