/
/
Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells
8,139 Views
•
15:29 min
•
August 05, 2016
DOI:
Summary
Automatically generated
Two assays for microscopy-based high-throughput screening of host factors involved in Brucella infection are described. The entry assay detects host factors required for Brucella entry and the endpoint assay those required for intracellular replication. While applicable for alternative approaches, siRNA screening in HeLa cells is used to illustrate the protocols.
Read Article
Cite this Article
Casanova, A., Low, S. H., Emmenlauer, M., Conde-Alvarez, R., Salcedo, S. P., Gorvel, J., Dehio, C. Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells. J. Vis. Exp. (114), e54263, doi:10.3791/54263 (2016).
Copy