/
/
Erzeugung und Identifizierung von GM-CSF Abgeleitet Alveolare ähnlichen Makrophagen und dendritische Zellen aus Knochenmark der Maus
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Erzeugung und Identifizierung von GM-CSF Abgeleitet Alveolare ähnlichen Makrophagen und dendritische Zellen aus Knochenmark der Maus
17,585 Views
•
11:05 min
•
June 25, 2016
DOI:
Summary
Automatically generated
Bone marrow cells cultured with granulocyte macrophage colony stimulating factor (GM-CSF) generate a heterogeneous culture containing macrophages and dendritic cells (DCs). This method highlights using MHCII and hyaluronan (HA) binding to differentiate macrophages from the DCs in the GM-CSF culture. Macrophages in this culture have many similarities to alveolar macrophages.
Read Article
Cite this Article
Dong, Y., Arif, A. A., Poon, G. F. T., Hardman, B., Dosanjh, M., Johnson, P. Generation and Identification of GM-CSF Derived Alveolar-like Macrophages and Dendritic Cells From Mouse Bone Marrow. J. Vis. Exp. (112), e54194, doi:10.3791/54194 (2016).
Copy