'Dergi'
/
Ormancılık ve botanik
/
Generation and Identification of GM-CSF Derived Alveolar-like Macrophages and Dendritic Cells From Mouse Bone Marrow
JoVE Journal
Ormancılık ve botanik
'Bu içeriği görüntülemek için JoVE aboneliği gereklidir.'  'Oturum açın veya ücretsiz deneme sürümünü başlatın.'
JoVE Journal Ormancılık ve botanik
Generation and Identification of GM-CSF Derived Alveolar-like Macrophages and Dendritic Cells From Mouse Bone Marrow

Generation and Identification of GM-CSF Derived Alveolar-like Macrophages and Dendritic Cells From Mouse Bone Marrow

DOI:
10.3791/54194-v

•

11:05 min

•

June 25, 2016

•

, , , , ,
1Microbiology and Immunology,University of British Columbia

'Bölümler'

  • 00:05'Başlık'
  • 01:11Murine Femur and Tibia Single Bone Marrow Suspesion Preparation
  • 05:55Bone Marrow Cell (BMC) Culture
  • 08:39Results: Representative Bone Marrow Dendritic Cell Culture Analyses
  • 10:13Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

Bone marrow cells cultured with granulocyte macrophage colony stimulating factor (GM-CSF) generate a heterogeneous culture containing macrophages and dendritic cells (DCs). This method highlights using MHCII and hyaluronan (HA) binding to differentiate macrophages from the DCs in the GM-CSF culture. Macrophages in this culture have many similarities to alveolar macrophages.

'Etiketler'

GM-CSFAlveolar MacrophagesDendritic CellsBone MarrowIn VitroMousePulmonary ProteinosisCell Isolation

Article

Embed

'ÇALMA LİSTESİNE EKLE'

Usage Statistics

'İlgili Videolar'

Toxoplasma gondii Cyst Wall Formation in Activated Bone Marrow-derived Macrophages and Bradyzoite Conditions

A Cell Free Assay System Estimating the Neutralizing Capacity of GM-CSF Antibody using Recombinant Soluble GM-CSF Receptor

Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies

Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Bone Marrow-derived Macrophage Production

Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow

Generation of Human Monocyte-derived Dendritic Cells from Whole Blood

Generation of Large Numbers of Myeloid Progenitors and Dendritic Cell Precursors from Murine Bone Marrow Using a Novel Cell Sorting Strategy

Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages

Read Article