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使用IL-1β启动子驱动的红色荧光蛋白记者小鼠中性粒细胞引发的活体成像
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Intravital Imaging of Neutrophil Priming Using IL-1β Promoter-driven DsRed Reporter Mice
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使用IL-1β启动子驱动的红色荧光蛋白记者小鼠中性粒细胞引发的活体成像

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09:34 min

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June 22, 2016

DOI:

10.3791/54070-v

DOI:
10.3791/54070-v

•

09:34 min
June 22, 2016

•

1 Views
, ,
1Department of Medical Microbiology and Immunology,University of Toledo College of Medicine and Life Sciences, 2Department of Internal Medicine,Yale University School of Medicine, 3Department of Pathophysiology,Southern Medical University (China)

Summary

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This current protocol employs fluorescent reporters, in vivo labeling, and intravital imaging techniques to enable monitoring of the dynamic process of neutrophil priming in living animals.

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Intravital ImagingNeutrophil PrimingIL-1β PromoterDsRed Reporter MiceFluorescent LabelingFlow CytometryAnesthesiaEar Thickness MeasurementOxazolone

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Cite this Article

Yao, Y., Liu, Y., Takashima, A. Intravital Imaging of Neutrophil Priming Using IL-1β Promoter-driven DsRed Reporter Mice. J. Vis. Exp. (112), e54070, doi:10.3791/54070 (2016).

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