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Intravital Imaging of Neutrophil Priming Using IL-1β Promoter-driven DsRed Reporter Mice
JoVE Journal
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JoVE Journal İmmünoloji ve Enfeksiyon
Intravital Imaging of Neutrophil Priming Using IL-1β Promoter-driven DsRed Reporter Mice

Intravital Imaging of Neutrophil Priming Using IL-1β Promoter-driven DsRed Reporter Mice

9,487 Views

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09:34 min

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June 22, 2016

DOI:

10.3791/54070-v

DOI:
10.3791/54070-v

•

09:34 min
June 22, 2016

•

1 Views
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1Department of Medical Microbiology and Immunology,University of Toledo College of Medicine and Life Sciences, 2İç Hastalıkları Anabilim Dalı,Yale University School of Medicine, 3Department of Pathophysiology,Southern Medical University (China)

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This current protocol employs fluorescent reporters, in vivo labeling, and intravital imaging techniques to enable monitoring of the dynamic process of neutrophil priming in living animals.

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Intravital ImagingNeutrophil PrimingIL-1β PromoterDsRed Reporter MiceFluorescent LabelingFlow CytometryAnesthesiaEar Thickness MeasurementOxazolone

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Yao, Y., Liu, Y., Takashima, A. Intravital Imaging of Neutrophil Priming Using IL-1β Promoter-driven DsRed Reporter Mice. J. Vis. Exp. (112), e54070, doi:10.3791/54070 (2016).

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