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Epigenetic Conversion as a Safe and Simple Method to Obtain Insulin-secreting Cells from Adult Skin Fibroblasts
Chapters
- 00:05Title
- 00:50Skin Fibroblast Isolation
- 01:53Fibroblast Culture and Plating for Epigenetic Conversion
- 04:00Increase Cell Plasticity Using the De-methylating Agent 5-aza-CR
- 05:31Pancreatic Differentiation Protocol
- 06:47Results: Morphological and Functional Changes During Epigenetic Conversion
- 08:02Conclusion
Here, a new method that allows the conversion of adult skin fibroblasts into insulin-secreting cells is presented. This technique is based on epigenetic conversion, does not involve the use of retroviral vectors nor the acquisition of a stable pluripotent state. It is therefore highly promising for translational medicine applications.
