Low density cultures of primary hippocampal neurons usually require glia feeder layer to supply neurotrophic factors and sustain longevity. We describe here a simplified method to culture ultra-low density neurons on glass coverslips in the presence of a high density neuronal feeder layer, which facilitates investigation of specific neuronal-autonomous mechanisms.
Lu, Z., Piechowicz, M., Qiu, S. A Simplified Method for Ultra-Low Density, Long-Term Primary Hippocampal Neuron Culture. J. Vis. Exp. (109), e53797, doi:10.3791/53797 (2016).