/
/
A Simplified Method for Ultra-Low Density, Long-Term Primary Hippocampal Neuron Culture
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
A Simplified Method for Ultra-Low Density, Long-Term Primary Hippocampal Neuron Culture
15,367 Views
•
11:19 min
•
March 05, 2016
DOI:
Summary
Automatically generated
Low density cultures of primary hippocampal neurons usually require glia feeder layer to supply neurotrophic factors and sustain longevity. We describe here a simplified method to culture ultra-low density neurons on glass coverslips in the presence of a high density neuronal feeder layer, which facilitates investigation of specific neuronal-autonomous mechanisms.
Read Article
Cite this Article
Lu, Z., Piechowicz, M., Qiu, S. A Simplified Method for Ultra-Low Density, Long-Term Primary Hippocampal Neuron Culture. J. Vis. Exp. (109), e53797, doi:10.3791/53797 (2016).
Copy