Presented here is a straightforward method for the isolation and flow cytometric analysis of glioma-infiltrating peripheral blood mononuclear cells that yields time-dependent quantitative data on the number and activation status of immune cells entering the early brain tumor microenvironment.
Baker, G. J., Castro, M. G., Lowenstein, P. R. Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells. J. Vis. Exp. (105), e53676, doi:10.3791/53676 (2015).