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Eşzamanlı İki foton<em> İn Vivo</em> Fare Retrosplenial kortekste sinaptik Girişlerin Görüntüleme ve postsinaptik Hedefler
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16:45 min
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March 13, 2016
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This video shows the craniotomy procedure that allows chronic imaging of neurons in mouse retrosplenial cortex using in vivo two photon microscopy in Thy1-GFP transgenic line. This approach is combined with injection of mCherry-expressing adeno-associated virus into dorsal hippocampus. These techniques allow long-term monitoring of experience-dependent structural plasticity in RSC.
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Łukasiewicz, K., Robacha, M., Bożycki, Ł., Radwanska, K., Czajkowski, R. Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex. J. Vis. Exp. (109), e53528, doi:10.3791/53528 (2016).
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