Méthodes de transfert localisé précisément de cellules ou de l'ADN dans des embryons de souris après l'implantation précoce

We demonstrate a method for grafting cultured cells into defined sites of early mouse embryos to determine their in vivo potential. We also introduce an optimized electroporation method that uses glass capillaries of known diameter, allowing the precise delivery of exogenous DNA into a few cells in the embryos.