/
/
Methods for Precisely Localized Transfer of Cells or DNA into Early Postimplantation Mouse Embryos
This content is Free Access.
Methods for Precisely Localized Transfer of Cells or DNA into Early Postimplantation Mouse Embryos
10,211 Views
•
09:04 min
•
December 25, 2015
DOI:
Summary
Automatically generated
We demonstrate a method for grafting cultured cells into defined sites of early mouse embryos to determine their in vivo potential. We also introduce an optimized electroporation method that uses glass capillaries of known diameter, allowing the precise delivery of exogenous DNA into a few cells in the embryos.
Read Article
Cite this Article
Huang, Y., Wilkie, R., Wilson, V. Methods for Precisely Localized Transfer of Cells or DNA into Early Postimplantation Mouse Embryos. J. Vis. Exp. (106), e53295, doi:10.3791/53295 (2015).
Copy