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방법은 작은 RNA를과의 리보솜 점유 규제 역할을 조사하기 위해<em> 말라리아 원충</em
Chapters
- 00:05Title
- 00:50Bio-tagging and Elution of miRNA-mRNA Fusion Products
- 03:02Polysome Separation to Determine the Ribosomal Occupancy of P. falciparum
- 08:34Results: Erythrocyte mRNA Enrichment and Parasite Polysomes
- 09:32Conclusion
Human microRNAs translocate from host erythrocytes to Plasmodium falciparum parasites. Here, the techniques used to transfect synthetic microRNAs into host erythrocytes and isolate all RNAs from P. falciparum are described. In addition, this paper will detail a method of polysome isolation in P. falciparum to determine the ribosomal occupancy and translational potential of parasite transcripts.
