This protocol describes a system architecture for performing automated small volume (0.15–1.5 ml) particle separations using a microfluidic device, and discusses methods to optimize acoustofluidic device performance and operation.
Fong, E. J., Huang, C., Hamilton, J., Benett, W. J., Bora, M., Burklund, A., Metz, T. R., Shusteff, M. A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice. J. Vis. Exp. (105), e53051, doi:10.3791/53051 (2015).