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Células T Captura bacterias por transinfection de células dendríticas
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JoVE 신문 면역학 및 감염병학
T Cells Capture Bacteria by Transinfection from Dendritic Cells
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Células T Captura bacterias por transinfection de células dendríticas

11,548 Views

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11:39 min

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January 13, 2016

DOI:

10.3791/52976-v

DOI:
10.3791/52976-v

•

11:39 min
January 13, 2016

•

1 Views
, , , ,
1Cellular and Molecular Biology, Spanish National Research Council (CSIC),Spanish National Biotechnology Centre (CNB-CSIC), 2Hospital de Santa Cristina,Healthcare Research Institute Princesa Hospital (IIS-Princesa)

Summary

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Aquí se presenta un protocolo para medir la captura bacteriana por las células T CD4 + que se produce durante la presentación de antígenos a través de transinfection de células dendríticas infectadas previamente (DC). Mostramos cómo realizar los pasos necesarios: el aislamiento de pilas, la infección de la formación de conjugado de células DC, DC / T, y la medición de bacterias transfección de células T.

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Cite this Article

Cruz-Adalia, A., Ramírez-Santiago, G., Torres-Torresano, M., Garcia-Ferreras, R., Veiga Chacón, E. T Cells Capture Bacteria by Transinfection from Dendritic Cells. J. Vis. Exp. (107), e52976, doi:10.3791/52976 (2016).

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