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Immunology and Infection

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JoVE Journal Immunology and Infection
T Cells Capture Bacteria by Transinfection from Dendritic Cells
 

T Cells Capture Bacteria by Transinfection from Dendritic Cells

Article DOI: 10.3791/52976-v • 11:39 min • January 13th, 2016
January 13th, 2016 •
1, 1,2, 1,2, 1,2, 1
1Cellular and Molecular Biology, Spanish National Research Council (CSIC), Spanish National Biotechnology Centre (CNB-CSIC), 2Hospital de Santa Cristina, Healthcare Research Institute Princesa Hospital (IIS-Princesa)

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Here a protocol is presented to measure bacterial capture by CD4+ T cells which occurs during antigen presentation via transinfection from pre-infected dendritic cells (DC). We show how to perform the necessary steps: isolation of primary cells, infection of DC, DC/T cell conjugate formation, and measurement of bacterial T cell transfection.

Tags

T Cells Bacteria Transinfection Dendritic Cells CD4+ T Cell Mouse Model Listeria Monocytogenes Salmonella Enteritidis Antigen Presentation Viral Infections Professional Phagocytes Adaptive Immunity Innate Immunity Immunology Protocol Mouse Model Esteban Veiga Guillermo Ramirez-Santiago Raquelle Garcia-Ferreras Monica Torres-Torresano Sterile Media Instruments Pipette Tips Culture Dishes Lipopolysaccharide
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