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qPCRTag Analysis – A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping
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May 25, 2015
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Designer chromosomes of the Synthetic Yeast Genome project, Sc2.0, can be distinguished from their native counterparts using a PCR-based genotyping assay called PCRTagging, which has a presence/absence endpoint. Here we describe a high-throughput real time PCR detection method for PCRTag genotyping.
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Mitchell, L. A., Phillips, N. A., Lafont, A., Martin, J. A., Cutting, R., Boeke, J. D. qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping. J. Vis. Exp. (99), e52941, doi:10.3791/52941 (2015).
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