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Generation og Multi-fænotypisk High-indhold Screening af<em> Coxiella burnetii</em> Transposon Mutants
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Generation og Multi-fænotypisk High-indhold Screening af<em> Coxiella burnetii</em> Transposon Mutants
Chapters
- 00:05Title
- 01:49Isolation of Individual Transposon Mutants
- 03:44Evaluation of Bacterial Concentration and Single Primer Colony PCR
- 05:34Eukaryotic Cell Challenge with Coxiella Mutants and Monitoring of Intracellular Growth
- 07:17Preparation of Samples for Automated Image Acquisition
- 09:25Results: Viability and Infectivity of Coxiella Transposon Mutants
- 11:14Conclusion
Coxiella burnetii er en obligat intracellulær Gram-negativ bakterie er ansvarlig for zoonotisk sygdom Q-feber. Her beskriver vi fremgangsmåder til generering af Coxiella fluorescerende transposon mutanter samt automatiseret identifikation og analyse af de resulterende internalisering, replikations- og cytotoksiske fænotyper.
