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生成和多表型高内涵筛选<em>贝氏柯克斯体</em>转座子突变体
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Generation and Multi-phenotypic High-content Screening of Coxiella burnetii Transposon Mutants
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生成和多表型高内涵筛选<em>贝氏柯克斯体</em>转座子突变体

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11:44 min

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May 13, 2015

DOI:

10.3791/52851-v

DOI:
10.3791/52851-v

•

11:44 min
May 13, 2015

•

1 Views
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1Cell Biology of Bacterial Infections, CNRS, FRE3689, CPBS,Université Montpellier

Summary

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贝氏柯克斯体是专性细胞内革兰阴性细菌负责对人畜共患病Q热。在这里，我们描述了用于Coxiella荧光转座子突变体的产生，以及自动识别和所得内化，复制和细胞毒性表型分析的方法。

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Martinez, E., Cantet, F., Bonazzi, M. Generation and Multi-phenotypic High-content Screening of Coxiella burnetii Transposon Mutants. J. Vis. Exp. (99), e52851, doi:10.3791/52851 (2015).

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