/
/
Automated Cell Enrichment of Cytomegalovirus-specific T cells for Clinical Applications using the Cytokine-capture System
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Automated Cell Enrichment of Cytomegalovirus-specific T cells for Clinical Applications using the Cytokine-capture System
12,242 Views
•
10:24 min
•
October 05, 2015
DOI:
Summary
Automatically generated
The goal of this protocol is to manufacture pathogen-specific clinical-grade T cells using a bench-top, automated, second generation cell enrichment device that incorporates a closed cytokine capture system and does not require dedicated staff or use of a GMP facility. The cytomegalovirus pp65-specific-T cells generated can be directly administered to patients.
Explore More Videos
Automated Cell EnrichmentCytomegalovirus-specific T CellsClinical ApplicationsCytokine-capture SystemAllogeneic Hematopoietic Stem-Cell TransplantationViral-specific T CellsGamma-interferonCytokine Capture System (CCS)CliniMACS ProdigyMagnetic Activated Cell SortingCMV Pp65-specific T CellsAutomated Cell Enrichment DeviceGMP FacilitiesBlood Banking Facilities
Read Article
Cite this Article
Kumaresan, P., Figliola, M., Moyes, J. S., Huls, M. H., Tewari, P., Shpall, E. J., Champlin, R., Cooper, L. J. Automated Cell Enrichment of Cytomegalovirus-specific T cells for Clinical Applications using the Cytokine-capture System. J. Vis. Exp. (104), e52808, doi:10.3791/52808 (2015).
Copy