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Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies
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Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies

Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies

19,213 Views

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07:29 min

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June 20, 2015

DOI:

10.3791/52801-v

DOI:
10.3791/52801-v

•

07:29 min
June 20, 2015

•

2 Views
, , , ,
1Stem Cells & Cancer Group, Molecular Pathology Program,Spanish National Cancer Research Center, 2Institute for Research in Biomedicine (IRB Barcelona), 3Center for Stem Cells in Cancer & Ageing, Barts Cancer Institute,Queen Mary University of London

Summary

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Pancreatic cancer stem cells (CSCs) can be expanded in vitro using the anchorage-independent sphere culture technique, which represents a powerful tool to study CSC biology and can serve as the first step to develop novel CSC-targeting therapies. Here the methodology for expanding, analyzing and targeting of pancreatic CSCs is provided.

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Cite this Article

Lonardo, E., Cioffi, M., Sancho, P., Crusz, S., Heeschen, C. Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies. J. Vis. Exp. (100), e52801, doi:10.3791/52801 (2015).

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