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Chapters
- 00:05Title
- 02:26vHipp-nAcc Synaptic Co-cultures
- 06:13FM1-43 Based Vesicular Fusion
- 08:12Calcium Imaging
- 09:35Results: Synaptic Co-culture of vHipp with nAcc allows Examination of Pre-synaptic Localization of nAChRs
- 10:57Conclusion
We developed a gene-chimeric preparation of ventral hippocampal – accumbens circuit in vitro that allows direct live imaging to analyze presynaptic mechanisms of nicotinic acetylcholine receptors (nAChRs) mediated synaptic transmission. This preparation also provides an informative approach to study the pre- and post-synaptic mechanisms of synaptic plasticity.
