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Live Imaging of Nicotine Induced Calcium Signaling and Neurotransmitter Release Along Ventral Hippocampal Axons
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Live Imaging of Nicotine Induced Calcium Signaling and Neurotransmitter Release Along Ventral Hippocampal Axons

Live Imaging of Nicotine Induced Calcium Signaling and Neurotransmitter Release Along Ventral Hippocampal Axons

8,873 Views

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12:19 min

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June 24, 2015

DOI:

10.3791/52730-v

DOI:
10.3791/52730-v

•

12:19 min
June 24, 2015

•

2 Views
, ,
1Department of Neurobiology and Behavior,Stony Brook University, 2Department of Pharmacological Science,Stony Brook University

Summary

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We developed a gene-chimeric preparation of ventral hippocampal – accumbens circuit in vitro that allows direct live imaging to analyze presynaptic mechanisms of nicotinic acetylcholine receptors (nAChRs) mediated synaptic transmission. This preparation also provides an informative approach to study the pre- and post-synaptic mechanisms of synaptic plasticity.

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Live ImagingNicotineCalcium SignalingNeurotransmitter ReleaseVentral HippocampusAxonsNicotinic Acetylcholine ReceptorsSynaptic PlasticityGene-chimeric PreparationVentral Hippocampus-accumbens CircuitGlutamatergic SynapsesPre-synaptic Nicotinic SignalingElectrophysiologyLive Cell Imaging

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Zhong, C., Talmage, D. A., Role, L. W. Live Imaging of Nicotine Induced Calcium Signaling and Neurotransmitter Release Along Ventral Hippocampal Axons. J. Vis. Exp. (100), e52730, doi:10.3791/52730 (2015).

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