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最大等长收缩力的测量通过透骨骼肌纤维生成

JoVE 신문
생체공학

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JoVE 신문 생체공학

Measurement of Maximum Isometric Force Generated by Permeabilized Skeletal Muscle Fibers

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最大等长收缩力的测量通过透骨骼肌纤维生成

24,784 Views

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11:30 min

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June 16, 2015

DOI:

10.3791/52695-v

DOI:

10.3791/52695-v

•

11:30 min

June 16, 2015

•

18 Views

Stuart M. Roche¹, Jonathan P. Gumucio^1,2, Susan V. Brooks^2,3, Christopher L. Mendias^1,2, Dennis R. Claflin^3,4

¹Department of Orthopaedic Surgery,University of Michigan Medical School, ²Department of Molecular & Integrative Physiology,University of Michigan Medical School, ³Department of Biomedical Engineering,University of Michigan Medical School, ⁴Department of Surgery, Section of Plastic Surgery,University of Michigan Medical School

Summary

化学剥皮，或透化，骨骼肌纤维的收缩特性的分析提供了强有力的手段，其中在单个肌细胞的水平来评估肌肉功能。在这篇文章中，我们勾勒出一个有效和可靠的技术准备和测试透骨骼肌纤维体外。

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