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Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence
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Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

15,257 Views

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11:49 min

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March 09, 2015

DOI:

10.3791/52612-v

DOI:
10.3791/52612-v

•

11:49 min
March 09, 2015

•

6 Views
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1Forensic Science Graduate Program, Biochemistry Track,University of Central Florida, 2Department of Chemistry,University of Central Florida, 3National Center for Forensic Science,University of Central Florida

Summary

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Here we describe an optimized and efficient removal strategy for the collection of bio-particles present in ‘touch DNA’ samples, together with an enhanced amplification protocol involving a one-step 5 µl micro-volume lysis/STR amplification, to permit the recovery of short tandem repeat (STR) profiles of the bio-particle donor(s).

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Forensic DNA AnalysisTouch DNAMicromanipulationSingle-source DNA ProfilesSTR AmplificationGenetic AnalysisBioparticle RecoveryEnhanced TechniquesTrace Biological Material

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Cite this Article

Farash, K., Hanson, E. K., Ballantyne, J. Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence. J. Vis. Exp. (97), e52612, doi:10.3791/52612 (2015).

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