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Parvis Growth Konkurrence Assay til Bestemmelse af Replication Fitness af Human Immunodeficiency Virus
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Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses

Parvis Growth Konkurrence Assay til Bestemmelse af Replication Fitness af Human Immunodeficiency Virus

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11:19 min

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May 04, 2015

DOI:

10.3791/52610-v

DOI:
10.3791/52610-v

•

11:19 min
May 04, 2015

•

2 Views
, , , , , , , , , , ,
1Department of Microbiology,University of Washington, 2Departments of Medicine and Laboratory Medicine,University of Washington, 3U.S Military HIV Research Program,Walter Reed Army Institute of Research, 4Henry M. Jackson Foundation

Summary

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Growth competition between nearly isogenic viruses provides a sensitive measurement for determining relative replication fitness. The protocols described here include the construction of recombinant HIV-1 clones, virus propagation and growth competition and analysis methods optimized to yield sensitive and consistent results.

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HIV-1Viral Replication FitnessIn Vitro Fitness AssayGrowth Competition AssayMultiplicity Of Infection (MOI)Viral DetectionRelative Fitness Calculation

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Manocheewa, S., Lanxon-Cookson, E. C., Liu, Y., Swain, J. V., McClure, J., Rao, U., Maust, B., Deng, W., Sunshine, J. E., Kim, M., Rolland, M., Mullins, J. I. Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses. J. Vis. Exp. (99), e52610, doi:10.3791/52610 (2015).

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