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Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses
JoVE Journal
免疫学と感染
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JoVE Journal 免疫学と感染
Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses

Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses

DOI:
10.3791/52610-v

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11:19 min

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May 04, 2015

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, , , , , , , , , , ,
1Department of Microbiology,University of Washington, 2Departments of Medicine and Laboratory Medicine,University of Washington, 3U.S Military HIV Research Program,Walter Reed Army Institute of Research, 4Henry M. Jackson Foundation

章

  • 00:05標題
  • 01:23Growth Competition
  • 05:00Determination of Viral Ratios
  • 09:16Results: HIV Replication Fitness Determined by Pairwise Growth Competition Assays
  • 10:54Conclusion

概要

自動翻訳

自動翻訳

Growth competition between nearly isogenic viruses provides a sensitive measurement for determining relative replication fitness. The protocols described here include the construction of recombinant HIV-1 clones, virus propagation and growth competition and analysis methods optimized to yield sensitive and consistent results.

タグ

HIV-1Viral Replication FitnessIn Vitro Fitness AssayGrowth Competition AssayMultiplicity Of Infection (MOI)Viral DetectionRelative Fitness Calculation

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