Journal
/
면역학 및 감염병학
/
Normal and Malignant Muscle Cell Transplantation into Immune Compromised Adult Zebrafish
JoVE 신문
면역학 및 감염병학
This content is Free Access.
JoVE 신문 면역학 및 감염병학
Normal and Malignant Muscle Cell Transplantation into Immune Compromised Adult Zebrafish

Normal and Malignant Muscle Cell Transplantation into Immune Compromised Adult Zebrafish

10,733 Views

•

09:39 min

•

December 26, 2014

DOI:

10.3791/52597-v

DOI:
10.3791/52597-v

•

09:39 min
December 26, 2014

•

3 Views
, , ,
1Molecular Pathology, Cancer Center and Center for Regenerative Medicine,Massachusetts General Hospital, 2Harvard Stem Cell Institute, 3GABBA – Instituto de Ciências Biomédicas Abel Salazar,Universidade do Porto

Summary

Automatically generated

Here, we present a protocol for cell transplantation of zebrafish skeletal muscle and embryonal rhabdomyosarcoma (ERMS) into adult immune compromised rag2E450fs homozygous mutant zebrafish. This protocol allows for the efficient analysis of regeneration and malignant transformation of muscle cells.

Explore More Videos

ZebrafishCell TransplantationSkeletal MuscleRhabdomyosarcomaRag2 MutantImmune CompromisedEngraftmentRegenerationCancer

Read Article

Cite this Article

Tenente, I. M., Tang, Q., Moore, J. C., Langenau, D. M. Normal and Malignant Muscle Cell Transplantation into Immune Compromised Adult Zebrafish. J. Vis. Exp. (94), e52597, doi:10.3791/52597 (2014).

Download .ris file

Copy

Share Video

.

Copy