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Induction de murin inflammation intestinale par transfert adoptif de effecteur CD4<sup> +</sup> CD45RB<sup> Haute</sup> T cellules dans des souris immunodéficientes
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Induction de murin inflammation intestinale par transfert adoptif de effecteur CD4<sup> +</sup> CD45RB<sup> Haute</sup> T cellules dans des souris immunodéficientes
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April 21, 2015
DOI:
1Center for Gastrointestinal Biology and Disease,University of North Carolina at Chapel Hill, 2Department of Microbiology and Immunology,University of North Carolina at Chapel Hill, 3Department of Genetics,University of North Carolina at Chapel Hill, 4Curriculum in Genetics and Molecular Biology,University of North Carolina at Chapel Hill
Summary
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Here, we present a protocol to induce colonic inflammation in mice by adoptive transfer of syngeneic CD4+CD45RBhigh T cells into T and B cell deficient recipients. Clinical and histopathological features mimic human inflammatory bowel diseases. This method allows the study of the initiation of colonic inflammation and progression of disease.
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Steinbach, E. C., Gipson, G. R., Sheikh, S. Z. Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice. J. Vis. Exp. (98), e52533, doi:10.3791/52533 (2015).
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