/
/
Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice
16,074 Views
•
08:37 min
•
April 21, 2015
DOI:
1Center for Gastrointestinal Biology and Disease,University of North Carolina at Chapel Hill, 2Department of Microbiology and Immunology,University of North Carolina at Chapel Hill, 3Department of Genetics,University of North Carolina at Chapel Hill, 4Curriculum in Genetics and Molecular Biology,University of North Carolina at Chapel Hill
Summary
Automatically generated
Here, we present a protocol to induce colonic inflammation in mice by adoptive transfer of syngeneic CD4+CD45RBhigh T cells into T and B cell deficient recipients. Clinical and histopathological features mimic human inflammatory bowel diseases. This method allows the study of the initiation of colonic inflammation and progression of disease.
Read Article
Cite this Article
Steinbach, E. C., Gipson, G. R., Sheikh, S. Z. Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice. J. Vis. Exp. (98), e52533, doi:10.3791/52533 (2015).
Copy