This content is Free Access.
Üç Boyutlu Kültürleri, mıkroRNA Analizi ve Putatif Hedef Genler üzerinde bir İnsan Sinir Kök Hücre Hattı Farklılaşma
9,597 Views
•
10:48 min
•
April 12, 2015
DOI:
Summary
Automatically generated
With the intent of characterizing changes in miRNAs on differentiated human neural stem cells (hNSCs) we describe hNSC differentiation on a three dimensional system,the evaluation of changes in microRNA expression by miRNA PCR array, and computational analysis for miRNA target prediction and its validation by dual luciferase assay.
Read Article
Cite this Article
Stevanato, L., Hicks, C., Sinden, J. D. Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes. J. Vis. Exp. (98), e52410, doi:10.3791/52410 (2015).
Copy