Journal
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发育生物学
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Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
JoVE 杂志
发育生物学
This content is Free Access.
JoVE 杂志 发育生物学
Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

DOI:
10.3791/52410-v

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10:48 min

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April 12, 2015

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, ,
1ReNeuron

Chapters

  • 00:05Title
  • 02:00Three Dimensional Culture of Human Neural Stem Cells Using 12-well Plates
  • 05:04Evaluation of Changes in miRNA Expression by miRNA PCR Array
  • 06:51Computational Analysis for miRNA Target Prediction
  • 09:01Results: Genes Targeted by miRNA are Identical from 3D Cultures of hNSCs
  • 10:23Conclusion

Summary

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With the intent of characterizing changes in miRNAs on differentiated human neural stem cells (hNSCs) we describe hNSC differentiation on a three dimensional system,the evaluation of changes in microRNA expression by miRNA PCR array, and computational analysis for miRNA target prediction and its validation by dual luciferase assay.

Tags

Human Neural Stem Cells3D CultureDifferentiationMicroRNATarget GenesSOX5NR4A3Axon OutgrowthMiRNA ExpressionComputational AnalysisDual Luciferase Assay

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