A streamlined approach to screening for the expression of recombinant membrane proteins in Escherichia coli based on fusion to green fluorescent protein is presented.
Bird, L. E., Rada, H., Verma, A., Gasper, R., Birch, J., Jennions, M., Lӧwe, J., Moraes, I., Owens, R. J. Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli. J. Vis. Exp. (95), e52357, doi:10.3791/52357 (2015).