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Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
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Biologia
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JoVE Journal Biologia
Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

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08:46 min

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January 06, 2015

DOI:

10.3791/52357-v

DOI:
10.3791/52357-v

•

08:46 min
January 06, 2015

•

29 Views
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1Oxford Protein Production Facility,Research Complex at Harwell, 2Protein Crystallography Group,Ruhr University, 3MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, 4Membrane Protein Laboratory,Diamond Light Source

Summary

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A streamlined approach to screening for the expression of recombinant membrane proteins in Escherichia coli based on fusion to green fluorescent protein is presented.

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Cite this Article

Bird, L. E., Rada, H., Verma, A., Gasper, R., Birch, J., Jennions, M., Lӧwe, J., Moraes, I., Owens, R. J. Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli. J. Vis. Exp. (95), e52357, doi:10.3791/52357 (2015).

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