Immortalized cancer cell lines can be grown as 3D cell cultures, a valuable model for biological research. This protocol describes mass spectrometry imaging of 3D cell cultures, including improvements in the sample preparation platform. The goal of this protocol is to instruct users to prepare 3D cell cultures for mass spectrometry imaging analysis.
Ahlf Wheatcraft, D. R., Liu, X., Hummon, A. B. Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models. J. Vis. Exp. (94), e52313, doi:10.3791/52313 (2014).