This article describes a method for the generation and propagation of human T cell clones that specifically respond to a defined alloantigen. This protocol can be adapted for cloning human T cells specific for a variety of peptide-MHC ligands.
Jama, B. P., Morris, G. P. Generation of Human Alloantigen-specific T Cells from Peripheral Blood. J. Vis. Exp. (93), e52257, doi:10.3791/52257 (2014).