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Generation of Human Alloantigen-specific T Cells from Peripheral Blood
JoVE Journal
免疫学と感染
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JoVE Journal 免疫学と感染
Generation of Human Alloantigen-specific T Cells from Peripheral Blood
DOI:

09:47 min

November 21, 2014

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  • 00:05標題
  • 01:27Isolation of T Cells from Whole Blood
  • 03:05Preparation of T Cells for Single-cell Flow Cytometry Cell Sorting
  • 05:20Culture and Expansion of Alloantigen-specific T Cell Clones
  • 07:50Results: Generation of Alloantigen-specific T Cells
  • 09:12Conclusion

概要

自動翻訳

This article describes a method for the generation and propagation of human T cell clones that specifically respond to a defined alloantigen. This protocol can be adapted for cloning human T cells specific for a variety of peptide-MHC ligands.

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