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Floral-Dip Transformation of Flax (Linum usitatissimum) to Generate Transgenic Progenies with a High Transformation Rate
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Floral-Dip Transformation of Flax (Linum usitatissimum) to Generate Transgenic Progenies with a High Transformation Rate

Floral-Dip Transformation of Flax (Linum usitatissimum) to Generate Transgenic Progenies with a High Transformation Rate

28,435 Views

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08:49 min

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December 19, 2014

DOI:

10.3791/52189-v

DOI:
10.3791/52189-v

•

08:49 min
December 19, 2014

•

14 Views
,
1Department of Biology,Case Western Reserve University

Summary

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Here, we present a protocol to transform flax using Agrobacterium-mediated plant transformation via floral-dip. This protocol is simple to perform and inexpensive, yet yields a higher transformation rate than the current available methods for flax transformation.

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Floral-dip TransformationLinum UsitatissimumAgrobacterium-mediated TransformationBinary Vector PlasmidLinum Insertion Sequence (LIS-1)High Transformation RateT1 SeedsDirect PCRPositive Transformants

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Bastaki, N. K., Cullis, C. A. Floral-Dip Transformation of Flax (Linum usitatissimum) to Generate Transgenic Progenies with a High Transformation Rate. J. Vis. Exp. (94), e52189, doi:10.3791/52189 (2014).

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