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Assessing Leukocyte-endothelial Interactions Under Flow Conditions in an Ex Vivo Autoperfused Microflow Chamber Assay
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Assessing Leukocyte-endothelial Interactions Under Flow Conditions in an Ex Vivo Autoperfused Microflow Chamber Assay

Assessing Leukocyte-endothelial Interactions Under Flow Conditions in an Ex Vivo Autoperfused Microflow Chamber Assay

9,388 Views

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09:01 min

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December 30, 2014

DOI:

10.3791/52130-v

DOI:
10.3791/52130-v

•

09:01 min
December 30, 2014

•

2 Views
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1Angiogenesis Laboratory, Department of Ophthalmology,Harvard Medical School, Massachusetts Eye & Ear Infirmary, 2St. Elizabeth’s Medical Center

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Leukocyte-endothelial InteractionsEx Vivo Autoperfused Microflow Chamber AssayInflammationPeripheral Blood Leukocytes (PBLs)Adhesion MoleculesIntravital MicroscopyFlow ConditionsLeukocyte Rolling VelocityImageJ

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Mulki, L., Sweigard, J. H., Connor, K. M. Assessing Leukocyte-endothelial Interactions Under Flow Conditions in an Ex Vivo Autoperfused Microflow Chamber Assay. J. Vis. Exp. (94), e52130, doi:10.3791/52130 (2014).

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