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Journal / Biology / Generation of Genomic Deletions in Mammalian Cell …
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Biology

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Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
 

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Article DOI: 10.3791/52118-v • 09:40 min • January 3rd, 2015
January 3rd, 2015 •
*1,2,3, *1, 1,2,3,4
1Harvard Medical School, 2Division of Hematology/Oncology, Boston Children's Hospital, 3Department of Pediatric Oncology, Dana-Farber Cancer Institute, 4Howard Hughes Medical Institute
* These authors contributed equally

챕터

요약
Automatic Translation

CRISPR/Cas9 is a robust system to produce disruption of genes and genetic elements. Here we describe a protocol for the efficient creation of genomic deletions in mammalian cell lines using CRISPR/Cas9.

Tags

Genomic Deletions Mammalian Cell Lines CRISPR/Cas9 Genome Engineering Genetic Perturbation CRISPR Design Cloning Delivery Double Strand Breaks Non-homologous End Joining (NHEJ) Repair Deletions Biallelic Modification Loss-of-function Studies Non-coding Elements
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