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A Guide to Modern Quantitative Fluorescent Western Blotting with Troubleshooting Strategies
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November 20, 2014
DOI:
1Division of Neurobiology, The Roslin Institute and Royal (Dick) School of Veterinary Studies,University of Edinburgh, 2Division of Developmental Biology, The Roslin Institute and Royal (Dick) School of Veterinary Studies,University of Edinburgh, 3Centre for Integrative Physiology,University of Edinburgh, 4Euan MacDonald Centre for Motor Neurone Disease Research,University of Edinburgh
Summary
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The advancement of western blotting using fluorescence has allowed detection of subtle changes in protein expression enabling quantitative analyses. Here we describe a robust methodology for detection of a range of proteins across a variety of species and tissue types. A strategy to overcome common technical problems is also provided.
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Eaton, S. L., Hurtado, M. L., Oldknow, K. J., Graham, L. C., Marchant, T. W., Gillingwater, T. H., Farquharson, C., Wishart, T. M. A Guide to Modern Quantitative Fluorescent Western Blotting with Troubleshooting Strategies. J. Vis. Exp. (93), e52099, doi:10.3791/52099 (2014).
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