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Measuring Local Anaphylaxis in Mice
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October 14, 2014
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Allergic responses, characterized by the activation of mast cells and basophils, are driven by the cross-linking of IgE and release of proinflammatory mediators. A quantitative assessment of allergic responses can be achieved by using Evans Blue dye to monitor changes in vascular permeability after allergen challenge.
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Evans, H., Killoran, K. E., Mitre, E. Measuring Local Anaphylaxis in Mice. J. Vis. Exp. (92), e52005, doi:10.3791/52005 (2014).
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