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Developmental Biology

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JoVE Journal Developmental Biology
Efficient Gene Transfer in Chick Retinas for Primary Cell Culture Studies
 

Efficient Gene Transfer in Chick Retinas for Primary Cell Culture Studies: An Ex-ovo Electroporation Approach

Article DOI: 10.3791/52002-v • 08:59 min • November 2nd, 2015
November 2nd, 2015 •
1, 1, 1
1Wilmer Eye Institute, The Johns Hopkins University School of Medicine

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요약
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Embryonic chick retinal cell cultures constitute valuable tools for the study of photoreceptor biology. We have developed an efficient gene transfer technique based on ex ovo plasmid electroporation of the retinas prior to culture. This technique considerably increases transfection efficiencies over currently available protocols, making genetic manipulation feasible for this system.

Tags

Gene Transfer Chick Retinas Primary Cell Culture Studies Ex-ovo Electroporation Approach Cone Photoreceptor-enriched Cultures Embryonic Chick Retinas Retinal Neurons Photoreceptors High Throughput Technologies Drug Development Genetic Manipulation Transfection Rate Plasmid Electroporation Technique Retinal Cells Plasmid-containing Solution Custom-made Electrodes Overexpression Studies Downregulation Of Gene Expression RNAi Technology Transgene Expression
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