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Preparation of Rat Serum Suitable for Mammalian Whole Embryo Culture
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Preparation of Rat Serum Suitable for Mammalian Whole Embryo Culture

Preparation of Rat Serum Suitable for Mammalian Whole Embryo Culture

DOI:
10.3791/51969-v

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11:48 min

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August 03, 2014

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, , ,
1Graduate School of Medicine,Jichi Medical University, 2Division of Biology, Center for Molecular Medicine,Jichi Medical University, 3Department of Developmental Neuroscience, United Center for Advanced Research and Translational Medicine (ART),Tohoku University School of Medicine

Chapters

  • 00:00Title
  • 00:03Introduction
  • 01:49Blood Collection
  • 05:26Rat Serum Preparation
  • 07:43Results
  • 08:13Troubleshooting 1: Non-separation of Layers
  • 08:39Troubleshooting 2: Mild Hemolysis
  • 09:16Conclusion

Summary

자동 번역

자동 번역

Mammalian whole embryo culture (WEC) is widely used in teratology and developmental biology. Immediately centrifuged rat serum is commonly provided as a medium for both mouse and rat WEC. In this video, we demonstrate our standard protocol for the preparation of high-quality rat serum suitable for mammalian WEC.

Tags

Rat SerumWhole Embryo CultureImmediately Centrifuged (IC) Rat SerumEx Vivo Embryo CultureBlood CollectionSerum ExtractionCentrifugationFibrin Clot Removal

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