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면역학 및 감염병학

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人THP-1巨噬细胞通过核转高效转染

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection

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人THP-1巨噬细胞通过核转高效转染

45,266 Views

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07:54 min

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September 02, 2014

DOI:

10.3791/51960-v

DOI:

10.3791/51960-v

•

07:54 min

September 02, 2014

•

42 Views

Marten B. Maeß¹, Berith Wittig¹, Stefan Lorkowski¹

¹Institute of Nutrition,Friedrich Schiller University Jena

Summary

这个协议提出通过电穿孔转染人THP-1巨噬细胞中的siRNA或质粒DNA具有高转染效率，同时保持高的细胞活力和分化和极化全细胞巨噬细胞的能力的有效和可靠的方法。

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