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Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection
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Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection
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September 02, 2014
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This protocol presents an efficient and reliable method to transfect human THP-1 macrophages with siRNA or plasmid DNA by electroporation with high transfection efficiency while maintaining high cell vitality and full macrophage capacity for differentiation and polarization.
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Maeß, M. B., Wittig, B., Lorkowski, S. Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection. J. Vis. Exp. (91), e51960, doi:10.3791/51960 (2014).
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