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ヤツメウナギ網様体軸索の急性解離は、個別の機能シナプス前終末のリリース顔膜から録音を有効にする方法
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October 01, 2014
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Recording Ca2+ currents at the presynaptic release face membrane is key to a precise understanding of Ca2+ entry and neurotransmitter release. We present an acute dissociation of the lamprey spinal cord that yields functional isolated reticulospinal axons, permitting recording directly from the release face membrane of individual presynaptic terminals.
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Ramachandran, S., Alford, S. Acute Dissociation of Lamprey Reticulospinal Axons to Enable Recording from the Release Face Membrane of Individual Functional Presynaptic Terminals. J. Vis. Exp. (92), e51925, doi:10.3791/51925 (2014).
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