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JoVE Journal Neuroscience
Acute Dissociation of Lamprey Reticulospinal Axons to Enable Recording from the Release Face Membrane of Individual Functional Presynaptic Terminals
 

Acute Dissociation of Lamprey Reticulospinal Axons to Enable Recording from the Release Face Membrane of Individual Functional Presynaptic Terminals

Article DOI: 10.3791/51925-v • 12:01 min • October 1st, 2014
October 1st, 2014 •
1, 1
1Biological Sciences, University of Illinois at Chicago

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Recording Ca2+ currents at the presynaptic release face membrane is key to a precise understanding of Ca2+ entry and neurotransmitter release. We present an acute dissociation of the lamprey spinal cord that yields functional isolated reticulospinal axons, permitting recording directly from the release face membrane of individual presynaptic terminals.

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Dissociation Lamprey Reticulospinal Axons Recording Release Face Membrane Functional Presynaptic Terminals Synaptic Transmission Ca2+ Influx Voltage-gated Calcium Channels Vesicle Fusion Neurotransmitter Release Spatial And Temporal Synchrony Electrophysiological Recording Presynaptic Ca2+ Entry Imaging Techniques Macroscopic Current Measurements VGCCs Central Synapses Calyx-type Synapse Chick Ciliary Ganglion Rat Calyces
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