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Lamprey Reticulospinal Aksonlar Akut Ayrılma Bireysel Fonksiyonel Presinaptik Terminalleri Yayın Yüz Membranından Kayıt Enable
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October 01, 2014
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Recording Ca2+ currents at the presynaptic release face membrane is key to a precise understanding of Ca2+ entry and neurotransmitter release. We present an acute dissociation of the lamprey spinal cord that yields functional isolated reticulospinal axons, permitting recording directly from the release face membrane of individual presynaptic terminals.
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Ramachandran, S., Alford, S. Acute Dissociation of Lamprey Reticulospinal Axons to Enable Recording from the Release Face Membrane of Individual Functional Presynaptic Terminals. J. Vis. Exp. (92), e51925, doi:10.3791/51925 (2014).
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